5-Nucleotidase/CD73 Antibody Summary
| Immunogen |
Recombinant protein encompassing a sequence within the center region of human CD73. The exact sequence is proprietary.
|
| Localization |
Cell membrane, GPI-anchor
|
| Predicted Species |
Bovine (91%). Backed by our 100% Guarantee.
|
| Isotype |
IgG
|
| Clonality |
Polyclonal
|
| Host |
Rabbit
|
| Gene |
NT5E
|
| Purity |
Immunogen affinity purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Expected cross reactivity based on sequence homology: Mouse (88%), Rat (88%).
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
|
| Preservative |
0.01% Thimerosal
|
| Concentration |
0.97 mg/ml
|
| Purity |
Immunogen affinity purified
|
Alternate Names for 5-Nucleotidase/CD73 Antibody
- 5 nucleotidase (CD73)
- 5-NT
- 5-NT
- 5Nucleotidase
- 5-Nucleotidase
- 5-nucleotidase, ecto (CD73)
- CD73 antigen
- CD73
- E5NT
- EC 3.1.3.5
- ecto-5-nucleotidase
- eN
- eNT
- NT
- NT55-nucleotidase
- NT5E
- NTE
- Purine 5-Prime-Nucleotidase
Background
Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]