4EBP1 Antibody Summary
| Immunogen |
Antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human 4E-BP1. The immunogen is located within the last 50 amino acids of 4E-BP1.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
EIF4EBP1
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
4E-BP1 antibody can be used for the detection of 4E-BP1 by Western blot at 2. 5 – 5 mg/ml.
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| Control Peptide |
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Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS
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| Preservative |
0.02% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for 4EBP1 Antibody
- 4EBP1
- 4E-BP1
- eIF4E-binding protein 1
- EIF4EBP1
- eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1
- eukaryotic translation initiation factor 4E-binding protein 1
- PHAS-I
- PHAS-IMGC4316
- Phosphorylated heat- and acid-stable protein regulated by insulin 1
Background
The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms. One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5-UTRs. Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferationl, at least in part by inhibiting the activity of TOR towards 4E-BP1.