Tes immune responses in ML-281 prostate cancer (data not shown). The IFN stimulated genes have been implicated in several cancers, including prostate cancer; however, what specific role they play in the different cancers and at what disease stage are currently unknown [37?2]. Interestingly, the IFN stimulated genes were not affected by androgen induction in LNCaP cells (data now shown); this could mean that TCTP may collaborate with other factors that are not regulated by androgens. Alternatively, since androgen induction of TCTP takes at least 48 h, longer time exposure to androgens may be needed to observe any effects on IFN pathway related genes. Further work is necessary for determining the possible consequence of IFN gene expression changes on PCa cell growth and viability. The secreted form of TCTP is well-studied in immune cells, where it has been shown to function as a histamine releasing factor, induce secretion of various interleukins, initiate distinct signal transduction events, and affect cell proliferation (reviewed in [16]). Since TCTP was earlier found in prostatic fluids [12], it was suggested to have a role in prostate function and in prostate cancer; however, there have not been any JI 101 studies to date which addressed the possible effect of rTCTP on prostate cancer cells. Consistent with the other data presented herein, and the function of TCTP in immune cells, we found that rTCTP increased colony formation in LNCaP cells (Figure 6). This indicates that the proliferative effects of secreted TCTP is not restricted to immune cells and is also applicable to prostate cancer cells. rTCTP has previously been implicated in the induction of distinct signal transduction pathways in immune cells [30,32]; it is therefore of interest to investigate whether this is also the case in prostate cancer cells. Further studies elucidating the molecular mechanisms behind these results are therefore warranted.TCTP in Prostate CancerTCTP was previously found to be expressed in normal prostate and prostate cancer cells [12,21]; it was also found to be further increased in castration resistant prostate cancer [21]. In line with these findings, we found a significant increase in TCTP expression in a TMA representing a collection of prostate cancer samples from various cancer stages compared with benign prostate (Figure 7). These data are consistent with earlier findings where TCTP was suggested to be involved in the process of initiation and progression of castration resistant prostate cancer [21]. Taken together, our data and earlier findings suggest that TCTP expression is relevant for human prostate cancer. TCTP may have a unique role in regulating inflammation and carcinogenesis processes thought to be tightly linked, making it a potential biomarker and a therapeutic target in prostate cancer.AcknowledgmentsWe thank Thomas Pretlow for the xenograft tumor samples and Drs Arcuri and del Vecchio for the TCTP antiserum. We also thank the members of the FS laboratory and Dr Wayne Murrell for discussions and critical reading of the manuscript.Author ContributionsConceived and designed the experiments: MK MLS FS. Performed the experiments: MK MLS SQ. Analyzed the data: MK MLS BR FS. Contributed reagents/materials/analysis tools: HW BR HD. Wrote the paper: MK MLS FS.
The regulation of the early phase of transcriptional elongation is used to control the expression of many genes. When this process fails it leads to death or severe defects during development and.Tes immune responses in prostate cancer (data not shown). The IFN stimulated genes have been implicated in several cancers, including prostate cancer; however, what specific role they play in the different cancers and at what disease stage are currently unknown [37?2]. Interestingly, the IFN stimulated genes were not affected by androgen induction in LNCaP cells (data now shown); this could mean that TCTP may collaborate with other factors that are not regulated by androgens. Alternatively, since androgen induction of TCTP takes at least 48 h, longer time exposure to androgens may be needed to observe any effects on IFN pathway related genes. Further work is necessary for determining the possible consequence of IFN gene expression changes on PCa cell growth and viability. The secreted form of TCTP is well-studied in immune cells, where it has been shown to function as a histamine releasing factor, induce secretion of various interleukins, initiate distinct signal transduction events, and affect cell proliferation (reviewed in [16]). Since TCTP was earlier found in prostatic fluids [12], it was suggested to have a role in prostate function and in prostate cancer; however, there have not been any studies to date which addressed the possible effect of rTCTP on prostate cancer cells. Consistent with the other data presented herein, and the function of TCTP in immune cells, we found that rTCTP increased colony formation in LNCaP cells (Figure 6). This indicates that the proliferative effects of secreted TCTP is not restricted to immune cells and is also applicable to prostate cancer cells. rTCTP has previously been implicated in the induction of distinct signal transduction pathways in immune cells [30,32]; it is therefore of interest to investigate whether this is also the case in prostate cancer cells. Further studies elucidating the molecular mechanisms behind these results are therefore warranted.TCTP in Prostate CancerTCTP was previously found to be expressed in normal prostate and prostate cancer cells [12,21]; it was also found to be further increased in castration resistant prostate cancer [21]. In line with these findings, we found a significant increase in TCTP expression in a TMA representing a collection of prostate cancer samples from various cancer stages compared with benign prostate (Figure 7). These data are consistent with earlier findings where TCTP was suggested to be involved in the process of initiation and progression of castration resistant prostate cancer [21]. Taken together, our data and earlier findings suggest that TCTP expression is relevant for human prostate cancer. TCTP may have a unique role in regulating inflammation and carcinogenesis processes thought to be tightly linked, making it a potential biomarker and a therapeutic target in prostate cancer.AcknowledgmentsWe thank Thomas Pretlow for the xenograft tumor samples and Drs Arcuri and del Vecchio for the TCTP antiserum. We also thank the members of the FS laboratory and Dr Wayne Murrell for discussions and critical reading of the manuscript.Author ContributionsConceived and designed the experiments: MK MLS FS. Performed the experiments: MK MLS SQ. Analyzed the data: MK MLS BR FS. Contributed reagents/materials/analysis tools: HW BR HD. Wrote the paper: MK MLS FS.
The regulation of the early phase of transcriptional elongation is used to control the expression of many genes. When this process fails it leads to death or severe defects during development and.