ssues. Interestingly, the set of cis eQTLs unique to hippocampus was enriched in genes from the gene ontology category involved in the “positive regulation of behavior”. The top 100 cis eQTLs in each tissue along with locations of their corresponding peak markers and minimum P values are provided in Additional file 1. The presence of a SNP within the 50mer probe sequence of the transcripts interrogated by the microarray might produce spurious false positive cis eQTLs due to a change in binding avidity. To investigate this possibility, we downloaded a list of 8,265,759 known SNPs from the Perlegen SNP Database http://mouse.cs.ucla. edu/mousehapmap and searched for each of these SNPs in the 25,697 probes on the Illumina microarray. Of the SNPs in this list, 3,841 probes contained at least one SNP. In the hippocampus, we observed 535 eQTLs with SNPs while 317 were expected proportionally. The Pyrroloquinolinequinone disodium salt web striatum also showed slight enrichment with 602 cis eQTLs exhibiting SNPs in probes with 372 expected. Although probe SNPs did increase the number of observed cis eQTLs, the proportion was <15%, suggesting that >85% of cis eQTLs do not have evidence of being artifacts due to polymorphism. Of course, other naturally occurring polymorphisms likely exist that are not contained in the Perlegen SNP database and could also lead to false positive associations. In the hippocampus, we mapped 481,099 trans eSNPs regulating a total of 5,325 unique probes, while in the striatum, we mapped trans 619,418 eSNPs regulating a total of 15,348 unique probes. Using a counting algorithm, we estimated these numbers corresponded to a total of 19,876 trans eQTLs in the hippocampus and 60,150 trans eQTLs in the striatum. Genome-wide probe/marker plots for each significant eSNP are provided in the Supplementary materials. Selected cis and trans eQTLs from each tissue are shown in Weighted gene correlation network analysis We looked at the large scale organization of gene coexpression networks in the hippocampus and striatum microarray datasets. Weighted gene co-expression network analysis is a data reduction method that groups genes into modules in an unsupervised manner based on self-organizing properties of complex systems. This method has been used in several recent systems genetics studies to reveal functional gene networks. We identified 30 modules in hippocampus containing 39 to 8,445 genes and 25 modules in the striatum containing PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/19799681 34 to 14,582 genes. The largest module in each tissue is the grey module which is reserved for genes that do not separate into any other modules. The hippocampus expression data organized into five more modules than the striatum. This finding could reflect a greater cellular heterogeneity of the hippocampus compared to the striatum, as module construction can tease apart patterns of differential expression in mixtures of cell types. There were other differences in co-expression networks between the two tissues. For instance the sienna3 module in the hippocampus was not preserved in striatum. This module was significantly enriched in neuropeptide hormone activity and oxygen binding indicating that these molecular classes may play important roles in hippocampal function. To evaluate the degree of module conservation across the hippocampus and striatum, we calculated Z scores for preservation of each module using the hippocampus as a reference. The Zsummary statistic encapsulates 
evidence that a network module is preserved between a reference and