The homogenate was centrifuged for one.5 minutes, and supernatant (cytoplasmic extracts) was saved frozen at 270 uC. Then twenty five ml of ice-cold nuclear extraction buffer was included to the pellets and incubated for thirty minutes with intermittent mixing
A a hundred mM stock of 5FU was prepared in complete DMSO and stored at 220 uC. MP-A08For therapy, the…