CXCR4 expression can be detected in Ishikawa cells at the amount of the two mRNA and protein, but its ligand CXCL12a is absent [27], hence enabling management above the concentration of CXCL12a for binding scientific studies
The remaining cells were pelleted once again for 5 min at 3006g, and resuspended in one hundred mM Tris-HCl, pH…