The resulting gels were possibly stained with Coomassie blue G250 according to Sambrook et al, detailed in [forty six] or transferred to PVDF membrane using the iBlot method (Invitrogen) according to manufacturer’s instructions
Immobilized pY RNA1-s2 was incubated with the indicated GST-RRM proteins and SDS-Website page adopted by Coomassie blue staining this demonstrates…