The ligated patch methylated or unmethylated constructs were directly transfected into SH-Sy5y (human neuronal cell line) cells without passing the plasmid through bacterial cloning to keep the state of methylation of the gria2 5′ region and unmethylated promoter region
BrdU staining for visualization of proliferating Pluripotincells soon after KA therapy. (A) BrdU incorporation [30] in the different regions of…