The markers utilized were GAPDH for the cytosolic fraction and histone H2B for the nuclear fraction.Validation of SILAC and mass spectrometry effects by Western-blotting and qRT-PCR of differentially expressed proteins in response to minimal doses of both equally Lf isoforms
The skill of MDA-MB-231 cells to pass via Matrigelcoated filters was measured by the Boyden chamber invasion assay as described…