The fungus experienced been pre supernatants discarded and the pellets dissolved in TE buffer (10 mM Tris- HCl, 1 mM EDTA, pH 7.four). Genomic DNA (every single 10 mg) was analyzed on one% agarose gel and visualized less than UV light-weight
An estrogen-inducible ectopic gene expression vector, pER8, was explained by Zuo et al. [seventy one]. This method displays efficient induction…